The remaining fungal extracts exhibited significant level of total phenolic and flavonoid content, antioxidant and antibacterial activity. Pdf antimicrobial and antioxidant activity of endophytic fungi. C for 36 days and were inoculated into ml flasks containing 200gms of unpolished rice, soaked with 200 ml distilled water autoclaved. Antioxidant compounds like total phenol and flavonoid were also determined. Endophytic fungi from nerium oleander l apocynaceae. Keywords antioxidant activity, crude extract, endophytic fungi, fusarium oxysporum, otoba gracilipes, tropical forest. Hence in the present study phomopsis amygdale, an endophytic fungus isolated from the mangrove plant, the isolated fungus was cultivated under submerged culture condition was evaluated for their antioxidant activity. The fungal endophytes were identified based on morphological characters. Moreover, endophytic fungi have significant host specificity and tissue specificity because different habitats will harbor different fungal groups unterseher et al.
Evaluation of antioxidant and antibacterial activities of endophytic. In vitro antioxidant activities of endophytic fungi. Thin layer chromatography tlc bioautography guided screenings were done to evaluate antibacterial and antioxidant activities. The grown endophytes were subcultured in a fresh pda medium to get a pure culture of it. In the current study, most of the endophytic fungi indicated antioxidant activity to some extent table 2. Thus, our results suggest other fungi from tropical ecosystems should be explored as potential sources of novel enzymes and other metabolites with bioactivity.
The current study is the continuation of our ongoing work on the exploitation of marine endophytic fungi for medicinal purposes. Molecular identification of bja1 shows high homology with lasiodiplodia venezuelensis. Antibacterial and antioxidant activities of endophytic fungi. The medium was extracted using ethyl acetate and determined its antioxidant activity. Antioxidant, antibacterial activity, total phenolic content, radical scavenging potentials. Diversity and characterization of endophytic fungi. The antioxidant activity of the endophytic fungi extracts was evaluated by the dpph, frap and. The aim of the present study was to synthesize silver nanoparticle using c. Antibacterial and antioxidant potential of endophytic. An endophytic penicillium oxalicum isolated from citrus limon. Antimicrobial activity, antioxidant activity, endophytic fungi, active metabolites.
Artanti n, tachibana s, kardono lb, sukiman h 2011 screening of endophytic fungi having ability for antioxidative and alphaglucosidase inhibitor activities isolated from taxus sumatrana. Influence of fungal endophyte infection on phenolic. To better utilize this resource, the diversity and antioxidant activity of endophytic fungi isolated from s. Biological activity and production of metabolites from amazon. After 3 d, the plates were observed everyday for the growth of endophytic fungi. The antioxidant capacities of the endophytic fungal cultures were significantly correlated with their total phenolic contents, suggesting that phenolics were also the major antioxidant constituents of the endophytes. This study shows that the endophytic fungi isolated. Shylaja gunasekaran, shoba sundaramoorthy, uma anitha, mythili sathiavelu, sathiavelu arunachalam.
Antidiabetic and antioxidant bioactivity of various potential. Tropical ecosystems hold an extremely diverse array of endophytic fungi, but their potential use still remains to be explored. Influence of fungal endophyte infection on phenolic content. The 1, 1diphenyl2picrylhydrazyl scavenging, reducing power, and total antioxidant assay were used to evaluate the antioxidant activity. The present study examined anti bacterial and antioxidant potential of endophytic fungi isolated from two mangrove plants. Antimicrobial and antioxidant activities of endophytic fungi. Hence endophytic fungal extracts rms01 and eal02 can be considered as potentially good antioxidants.
In vitro antioxidant activities of endophytic fungi isolated. Here, we found that the crude extract of endophytic a. Screening of endophytic fungal secondary metabolites from garcinia kola and cola nitida for antioxidant properties j pharm res garcinia kola and cola nitida for antioxidant properties nwobodo dc 1, ihekwereme cp 2, ugwu mc 1 and okoye fbc 3. Diversity and antioxidant activity of culturable endophytic fungi from alpine plants of rhodiola crenulata, r. This study aimed to explore the antioxidant potential and antiviral activity of endophytic fungi which were isolated from healthy living. The aim of the present study was to evaluate antioxidant, antiangiogenic as well as wound healing potential of the endophytic fungus isolated from the seaweed sargassum wightii.
Endophytic fungi generally exist in the intercellular space of healthy plant tissues for all or part of their life cycle and have a close symbiotic relationship with the host plant arnold, 2007. Pdf in vitro antioxidant activity and total phenolic. Total antioxidant activity the total antioxidant activity of the endophytic extracts was measured spectrophotometrically by phosphomolybdenum method. The aim of the present study was to evaluate antioxidant, antiangiogenic as well as wound healing potential of the endophytic fungus isolated from the.
However, no information is available on whether such a relationship also exists in their. Screening of endophytic fungal secondary metabolites from. Antibacterial and antioxidant activities of endophytic fungi extracts of. Apr 01, 2007 the antioxidant capacities of the endophytic fungal cultures were significantly correlated with their total phenolic contents, suggesting that phenolics were also the major antioxidant constituents of the endophytes. This study aimed to explore the antioxidant potential and antiviral activity of endophytic fungi which were isolated from healthy living tissues of medicinal plants. By definition, endophytic fungi are the fungi that spend a part or whole of their life cycle. Antibacterial and antioxidant potential of endophytic fungi. Biological activity and production of metabolites from. Antioxidant activities of scapania verrucosa material and methods isolation of endophytic fungi all endophytic fungi were isolated from the s. Antiangiogenic, wound healing and antioxidant activity of. This study aimed at isolating endophytic fungi from citrus limon l.
Medicinal plants and their endophytes are important resources for discovery of natural products. A total of 110 endophytic fungi isolates were grouped into 8 genera on the basis of morphological characteristics, and csaproducing fungi were screened by liquid. Nevertheless, endophytic microorganisms can be pathogens, commensals, or mutualists, depending on plantendophyte interactions. In this study, novel endophytic fungi producing cajaninstilbene acid csa from pigeon pea cajanus cajan l. Characterization, antioxidant and antimicrobial activity of. In addition, we identified major bioactive principles in the samples by lcms and gcms. Antioxidant activity and phenolic compounds of endophytic. Endophytic fungi, metabolites, medicinal plants, antioxidant activity, phenolic compounds, chinese medicinal plants, traditional chinese medicine, tcm. Characterization, antioxidant and antimicrobial activity. The research will be helpful for searching new effective antioxidants and antibiotics from endophytic fungi associated with this medicinal plant. Also, these fungal endophytes may also be contributors to the nutritive value of the strawberry fruits. A total of 35 healthy samples whole plant, 1015 mm long were selected from 125 s. Staphylococcus aureus, klebsiella pneumoniae and salmonella enteritidis. Endophytic fungi were screened for antioxidant activity using 2,2.
However, no information is available on whether such a relationship also exists in their endophytic fungal metabolites. If you have a user account, you will need to reset your password the next time you login. Recently we have reported the antiangiogenic activity and synthesis of gold nanoparticles using c. Isolation of endophytic fungi from thermopsis lanceolata and their antioxidant activity 29 name of genus fungi number root stem leaf alternaria 5 3 2 aspergillus 2 2 0 chaetomium 1 0 0 fusarium 1 0 0 totalpercentage 956. Endophytic fungi from marine seaweeds are the less studied group of organisms with vast medical applications. Diabetes mellitus is a degenerative disease characterized by hyperglycemia due to insulin insulin deficiency either absoluteor relative. The aim of the present study is to evaluate antioxidant and antiangiogenic activity of ethyl acetate extract of the endophytic fungi, penicillium citrinum isolated from sargassum wightii. Bioactive compound analysis and antioxidant activity of. Research open access the endophytic fungi of salvia. Antiviral and antioxidant potential of fungal endophytes of. Antimicrobial, antioxidant activity and phytochemical. Endophytic fungi from marine seaweeds are less studied and presented as a new subject of investigation of novel therapeutic agents. Introduction nowadays, drug resistant microorganisms and free radical agents are gaining more attention.
The principle involved in this method is the reduction of molybdenum mo iv to mo v by the extracts, and the final greencolored mo v end product is measured as absorption at 695 nm. The potential antimicrobial and antioxidant activity of these myconanoparticles was also studied. Diversity and antioxidant activity of culturable endophytic fungi. The results showed that 18 isolates of endophytic fungi have antimalarial activity and one isolate inactive and also all isolates have antioxidant activity. Antioxidant activities of fungal endophytes isolated from. Endophytic strains 29 different taxa were isolated from 18 egyptian medicinal plants collected from saint katherine protectorate, egypt. Some of the endophytes were found to produce metabolites possessing strong antioxidant activities. Isolation and identification of flavonoidproducing.
Antimicrobial and antioxidant activity of endophytic fungi isolated from ethnomedicinal plants of the sacred forests of meghalaya, india. To evaluate the total phenolic content and total antioxidant capacity of ethyl acetate extracts of 21 endophytic fungi isolated from five sudanese medicinal plants. Five isolates rct45, rct63, rct64, rac76, and rsc57 with strong antioxidant activities were isolated from rhodiola plants with high antioxidant capacities. Dpph free radical scavenging method was used to determine in vitro antioxidant activity. In this study, we isolated an endophytic fungus from the leaves of otoba gracilipes, a medicinal tree from a tropical rainforest in colombia. These isolates were aspergillus awamori dt11 and corynespora cassiicola dt. Lolium perenne is a major forage and turf grass, which is often naturally infected with a wildtype strain ewt of the fungal endophyte neotyphodium lolii, establishing a symbiotic relationship. Endophytic fungi exhibiting broad spectrum of antimicrobial activity were subjected for the production of secondary metabolites. The fresh mycelia of endophytic fungi were grown on pda plates at 282. Antidiabetic and antioxidant bioactivity of various. There were six extracts with mic value of endophytic fungi have been identified to show antioxidant activity. L ethyl acetate extract of endophytic fungi metabolite and methanolic plant extract at three different concentrations 2. Bioactive molecules from new sources, such as endophytic fungi, deserve attention because they may lead to drugs with di erent pathologies,food additives, or cosmetics.
The bioactive compounds from endophytic fungi possess antimicrobial,38 antioxidant,38,39 immunomodulating, and anticancer activities. A total of 14 endophytic fungi were isolated from different parts of s. Endophytic fungi were identified by standard mycology manuals by ellis 1971, sutton 1980, barnett and hunter 1987 and domsh et al. Secondary metabolites produced by endophytic fungi have a broad spectrum of bioactivity, such as for antimicrobial, antioxidant, antitumor, enzymatic and surfactant compounds canuto. Diversity and antioxidant activity of culturable endophytic. Materials and methods isolation of endophytic fungi. Screening of endophytic fungal secondary metabolites from garcinia kola and cola nitida for antioxidant properties j pharm res screening of endophytic fungal secondary metabolites from.
Endophytic fungi were isolated from healthy leaves of c. The purpose of this study was to determine the antioxidant activity, total phenolic, and flavonoid contents of the extract of endophytic fungi which were derived from the bogororiginated turmeric leaves via in vitro isolation. In vitro antioxidant and antibacterial activity of. Antioxidant activity test showed that ethyl acetate extract of endophytic fungi bja1 has the highest value. Characterization of the plant growthpromoting activities. As a negative control, cells were treated with only eagles mem with 10. Plant endophytic fungi are novel reservoir of bioactive compounds. Antibacterial and antioxidant activities of endophytic. In vitro antioxidant activity and total phenolic content of endophytic fungi isolated from eugenia jambolana lam.
Several previous studies have found a positive correlation between total antioxidant capacity tac and total phenolic content tpc of many medicinal plant extracts. Antiviral and antioxidant potential of fungal endophytes. Diversity and characterization of endophytic fungi isolated. In vitro antioxidant activity and phytochemical screening of endophytic extracts of crotalaria pallida free radicals and antioxidants 81 vol 1, issue 3, julsep, 2011. Pdf endophytic fungi from nerium oleander l apocynaceae. Biogenesis of silver nanoparticles using endophytic fungus. In this study, the impacts of different strains wild type ewt, ar1 ear1 and ar37 ear37, of n.
The aim of the present study was to test the antioxidant and cytotoxic and dna protection activities of ethyl acetate extracts of endophytic fungi isolated from tragia involucrata linn. Aspergillus fumigatus is commonly found in moist environments, negatively impacting yields and quality of crops 20. A comparative study on the antioxidant activity of. A coumarin analogue nfa from endophytic aspergillus. For inducing sporulation, each of the isolated fungal. Antioxidant activity in endophytic fungi isolated from gynostemma.
In vitro antioxidant and antibacterial activity of endophytic. These fungi colonize the healthy tissues of host plants. In vitro antioxidant activity and phytochemical screening. Antimicrobial activity and phytochemical analysis of crude. Endophytic fungi, metabolites, medicinal plants, antioxidant activity, phenolic. Of the seven endophytic fungi isolated, four were found to exhibit anti bacterial activity. This study was conducted to isolate endophytic fungi from plant twigs mahogany swietenia macrophylla king which active as antidiabetic and antioxidant. Among these three isolates also showed anti oxidant activity. Antimicrobial and antioxidant activities of endophytic. Antiangiogenic and antioxidant activity of endophytic fungus. Many endophytic fungi have been identified to show antioxidant activity. Possibly, these fungal endophytes contribute to the total antioxidant system of the plant. Endophytic fungi with antioxidant activity were screened and identified from gynostemma pentaphyllum, and gynostemma pentaphyllum saponin gp was used as. Research article chemical assessment and antimicrobial and.
Bioactive metabolites from an endophytic fungus penicillium. Following isolation and cultivation, we evaluated its extracellular crude extract for antioxidant activity. In vitro antioxidant activity and phytochemical screening of endophytic extracts of crotalaria pallida free radicals and antioxidants 81 vol 1, issue 3. Jul 12, 2019 recent studies have shown that endophytic fungi synthesize eps involved in plantendophyte interactions and that such biopolymers are characterized by structures that exhibit antioxidant activity chen et al. A pilot study of antioxidant potential of endophytic fungi from some. Antioxidant and cytotoxic potential of endophytic fungi. C ollection, identification and mass culture of endophytic fungi, cladosporium uredinicola from calophyllum tomentosum. Antiangiogenic and antioxidant activity of endophytic. Antioxidant and antibacterial activity of endophytic fungi.
An endophytic penicillium oxalicum isolated from citrus. Antioxidant, anti diabetic, anti hiv and anti alzheimer. The present study has been designed to investigate the antimicrobial and antioxidant potential of endophytic fungus nigrospora sp. Pdf antioxidant activity of syzygium samarangense l. Pestacin 1,3dihydroisobenzofuran and isopestacin isobenzofuranone bioactive compound obtained from an endophytic fungus such as p. Antioxidant activity of endophytic fungi isolated from. The antibacterial activity of the endophytic fungi extracts was tested against six human pathogenic strains, being three strains atcc and three hospital. In vitro antioxidant activity and phytochemical screening of.
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